| 초록 |
In this study, we report the use of a combination of restriction and ligation enzymes to synthesize DNA containing triblock copolymers. The synthesized triblocks have all blocks connected by covalent bonds, thus show superior stability against environmental factors that can denature DNA. Furthermore we introduce multiple cloning site (MCS) into the DNA triblock copolymer. We show that these restriction sites can be cut by their corresponding restriction enzymes, generating diblock copolymers with predetermined restriction ends. These regenerated diblocks can then be reconnected with other diblocks containing complementary restriction ends, allowing the generation of a variety of triblock structures with different DNA center block sequences as well as different synthetic end blocks. This study shows that by utilizing a single platform based on MCS, a variety of triblocks can be prepared through combination of just a few DNA diblock precursors. |
