| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
우수논문(구두)_석사과정 |
| 제목 |
Selection of affinity peptides as molecular binder for detection of cholera toxin |
| 초록 |
Cholera toxin is a major virulent agent of V. cholerae, and it can rapidly lead to severe dehydration causing death within few hours without appropriate clinical treatments. In this study, we present a method wherein unique and short peptides that bind to cholera toxin subunit B (CTXB) were selected through M13 phage display. The phage-displayed peptides analyzed using ELISA, were found to show specific affinities towards CTXB. We designed series of amino acids and immobilized them to the gold sensor layer using a peptide with a flexible link. Performance of the biosensor was studied using plasmonic-based optical techniques: localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS). The limit of detection (LOD) obtained by LSPR with 3σ-rule was 1.89 ng/mL, while SERS had a LOD of 3.51 pg/mL. In both cases, the sensitivity was much higher than the previously reported values, and our sensor system was specific towards actual CTXB secreted from V. cholera. |
| 저자 |
임종민1, 오서영2, 허남수2, 김지홍1, 조채환1, 류명이1, 박종필1, 허윤석2
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| 소속 |
1대구한의대, 2인하대 |
| 키워드 |
Cholera toxin; biosensor; peptide; LSPR; SERS
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| E-Mail |
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