| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 |
15권 1호, p.392 |
| 발표분야 |
생물화공 |
| 제목 |
A novel protease activity assaying method through signal amplification with oligonucleotides on gold nanoparticles |
| 초록 |
A sensitive protease assaying method is key tool for elucidating protease function, and developing therapeutics because of implications in the pathogenesis of diverse disease such as viral and bacterial infections and cancer and neurodegenerative diseases. Also it has been reported that secreted amount of proteases are related to cancers. Activity of proteases is assayed by measuring amount of digested substrates.Due to feasibility for developing high throughput assaying formats, synthetic short peptides are preferred. For developing fast and sensitive protease assaying methods, also nanoparticles such as quantum dots, gold nanoparticles and magnetic nanoparticles have been utilized. The reported methods show relatively low detection limits. However still these techniques require long assaying times (more than 12 hours) for acquiring enough detecting signals. Therefore, I propose a more fast and sensitive way to assay activity of protease by incorporating a signal amplification method. More detail working principle and detection limit of our protease assaying method will be presented. |
| 저자 |
김중현1, 정봉현2
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| 소속 |
1한국생명공학(연), 2바이오나노센터 한국생명공학(연) |
| 키워드 |
Protease; Matrix Metalloproteinase; Gold nanoparticle; Peptide; DNA; RNA; RNase H; Protease activity assay.
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| E-Mail |
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| 원문파일 |
초록 보기 |
