| 학회 | 한국공업화학회 |
| 학술대회 | 2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 | 8권 2호 |
| 발표분야 | 생물공학 |
| 제목 | Identification of cytosolic proteins of Streptomyces peucetius ATCC27952 using2D-gel electrophoresis and mass spectrometry |
| 초록 | Proteomics relying on two-dimensional polyacrylamide gel electrophoresis followed by protein identification with mass spectrometry is a promising experimental tool for physiological studies of microorganism. Streptomyces peucetius ATCC27952 is a doxorubicin(used broadly in chemotherapy) producing microorganism, and industrially useful. Here we report our own 2D reference map of Streptomyces peucetius ATCC27952 constructed by using isoelectric focusing followed by two-dimensional polyacrylamide gel electrophoresis. Protein identification was carried out by peptide tandem mass spectra using u-LC ESI-Tandem mass spectrometry. The two-dimensional gel pattern of proteins generated from any given cell system provides a fingerprint that is unique to those cells. This 2D reference map is applied to the study of antibiotics production regulation and doxorubicin resistance mechanism. |
| 저자 | 이광원1, 단기순1, 김병기1, 송재경2, 류광경2, 이희찬2 |
| 소속 | 1서울대, 2선문대 |
| 키워드 | streptomyces |
