| 초록 |
One of the main objectives of bone tissue engineering is the development of a scaffold system which helps to induce new bone regeneration without any complication. There are many materials used as scaffold, among them a new granular bone substitute containing 60wt% Hap & 40wt% TCP is showing satisfactory biocompatibility and osteoconductivity in clinical application. Different types of this cylindrical shape granular bone substitutes are commercially available with different diameter and height. They own different mechanical and morphological characteristics due to the difference in size, surface area, pore size and porosity. The aim of this study is to find out best formulation from these individual types of granular bone substitutes by combining in different proportion to optimize the performance of bone tissue regeneration. We evaluated two types of granular bone substitutes having 1mm & 3mm diameter and combination of these two in 1:3(1mm:3mm)wt% & 1:1(1mm:3mm)wt% and compared their different morphological, mechanical, in vitro and in vivo biocompatibility characteristics. Porosity and compressive strength of individual granules and in bulk were determined to evaluate the performance in clinical settings. The initial biocompatibility was examined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay with MC3T3E1osteoblst-like cells. In vivo investigation in rabbit model showed formation of bone within pore networks of different granular bone substitutes after 4 weeks and 8 weeks of implantation, which was examined by microcomputed tomography analysis and histological staining. |
