| 초록 |
Formate is an environmental and sustainable C1 feedstock, which is soluble and could be synthesized from CO2 or CO. In this study, we produced lycopene from formate using Methylorubrum extorquens by engineering non-mevalonoate pathway, also called MEP pathway, for effective lycopene production. First, LMZ01 strain was constructed by deleting CrtCD, genes for consuming lycopene, and used as a parental strain, showing improved lycopene production. Then, dxs, a rate-limiting step of MEP pathway, was overexpressed under lac, tac, and mxaF promoter, respectively. Expression level was shown to be strong in the order of mxaF, tac, and lac promoter by expressing EGFP. Interestingly, while the mxaF promoter with strong expression level was ineffective, the tac promoter with moderate level showed 45% increase in lycopene production. This showed lycopene could be produced reasonably from formate and suitable expression level of MEP pathway is crucial. |
