| 초록 |
Cell sheet technology has developed as a versatile cell harvesting method to yield contiguous cell monolayers robustly held together with cell junctions and endogenous extracellular matrix. Over 15 years of clincal data using autologous-sourced cell sheets have shown enhanced therapeutic properties through increased cell retention at target tissues. Recently, several allogeneic cell sheet therapies have also been reported using mesenchymal stem cell (MSC) sheets. However, optimized MSC sheet fabrication conditions have not yet been reported. In this study, we demonstrate crucial conditions for MSC sheet fabrication. Additionally, MSC sheets prepared with the optimized conditions easily graft spontaneously onto target site with no suture. Furthermore, MSC sheets recruited blood vessel formation and secreted human cytokine continuously in vivo. MSC sheet fabrication conditions described here should provide standardized allogeneic cell sheets functionality for future clinical studies. |
