| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Gene transfection efficacy of a ternary complexes with pDNA, Chitosan-g-PEI and Hyaluronic acid against CD44-receptor overexpressed cancer cells |
| 초록 |
Branched polyethyleneimine (bPEI) was a high gene transfection agent. However, its utilization as gene carrier was limited because of high cytotoxicity. To overcome these obstacle, chitosan was introduced to bPEI by EDC as coupling agent. The chemical structure of bPEI-grafted chitosan (CSPEI) was analyzed by 1H-NMR. The ternary complexes with pDNA, CSPEI, and hyaluronic acid (HA) was prepared by electrostatic charge interaction in aqueous phase. Also, its gelretardation assay showed that pDNA was completely bound by CSPEI and HA. In addition, pDNA from ternary complexes was protected against DNase. Moreover, the particle size and morphology of ternary complexes was confirmed by DLS and TEM. To prove a targeting effect of ternary complexes, competition assay was accomplished in HCT116 cell. These results suggest that it can be enhance to efficacy of gene delivery in CD44-receptor overexpressed cancer cell. (No. NRF-2014R1A2A1A10053027). |
| 저자 |
홍웅길1, 정경원1, 안준혁1, 남충현1, 박준규2, 나재운1
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| 소속 |
1순천대, 2(주)시지바이오 |
| 키워드 |
Hyaluronic acid; Ternary complexes; targeted CD44-receptor; Transfection;
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| E-Mail |
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