| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Development of high-throughput screening method for rapid selection of highly photo-inducible Haematococcus mutant |
| 초록 |
For enhancing astaxanthin production from Haematococcus pluvialis, various efforts have been made to find astaxanthin-hyperproducing mutants. However, the development and actual exploitation of high-throughput screening for selecting the improved strains has not been common in H. pluvialis research field. Herein, we propose a novel approach for simple and rapid selection of highly photo-inducible Haematococcus mutants’ library through serially combined method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Azide promptly triggered astaxanthin biosynthesis in H. pluvialis within 3 days by imposing artificial oxidative stress and oil-based extraction provided reliable and fast analytic method of the astaxanthin content without complex pretreatment. This universal screening method which accelerates both upstream and downstream of astaxanthin quantification would be useful for isolating the highly astaxanthin-producing microalgae. |
| 저자 |
홍민의1, 장원석2, 심상준1
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| 소속 |
1고려대, 2한국지역난방공사 미래개발원 |
| 키워드 |
Haematococcus pluvialis; Autotrophic astaxanthin production; highly photo-inducible mutant; high-throughput screening; Azide-based colorimetric assay; Oil-based astaxanthin extraction method
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| E-Mail |
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