| 초록 |
Cellular dynamics under complex topographical microenvironments is important for development and diseases, but systematic investigation has been limited due to lack of technology. To address this problem, we developed a new dynamic cell patterning method based on a cell-friendly photoresist polymer PDMP. PDMP thin films were casted on nanostructured surfaces by spincoating. Upon UV exposure, PDMP thin films become soluble in near-neutral aqueous buffers with minimal cytotoxicity. By performing series of microscope projection photolithography (MPP) on PDMP thin films and sequentially depositing cells on nanostructured surfaces, we could successfully create single cell arrays of adhering cells on nanostructured surfaces. Then, dynamic cellular processes such as cell spreading and cell migration were triggered by removing PDMP thin films surrounding cells by UV illumination. Using this new method, we quantitatively studied spreading dynamics of cells on complex nanostructured surfaces. |
