| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터) |
| 권호 |
44권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Highly sensitive detection system of pandemic influenza A (H1N1) virus infection with specific microRNA as a biomarker |
| 초록 |
Recently, several miRNAs have been reported to be closely related to influenza A virus infection, replication and immune response. Therefore, the development of an infectious disease detection system using these miRNAs as biomarkers is actively underway. Herein, we identified two miRNAs (miR-181c-5p and miR-1254) as biomarkers for pandemic influenza A H1N1 (pH1N1) virus infection and proposed the catalytic hairpin assembly (CHA)-based in vitro diagnostic (CIVD) system for highly sensitive diagnosis; this system is composed of two sets of cascade hairpin probes for detecting miR-181c-5p and miR-1254. We demonstrated that the CIVD kits could not only detect subnanomolar levels of target miRNAs but also distinguish even single base mismatches. Moreover, the proposed CIVD system has shown excellent detection performance in real cellular RNA samples and is in agreement with the results of microarray and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. |
| 저자 |
임재우1, 임은경1, 이도경2
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| 소속 |
1한국생명공학(연), 2바이오나노헬스가드연구단 |
| 키워드 |
Influenza A virus (IAV); in vitro diagnostics (IVD); microRNA; biomarker; catalytic hairpin assembly (CHA); enzyme-free signal amplification
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| E-Mail |
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