| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Artificial primary-miRNAs as a platform for simultaneous delivery of siRNA and antisense oligonucleotide for multimodal gene regulation |
| 초록 |
Here we report self-assembled primary microRNA mimics for achieving simultaneous multimodal gene regulation. Primary microRNA mimics were designed as substrate RNAs to recruit and interact with Drosha/DGCR8 microprocessor. Incorporation of functional RNA motifs and site-specific chemical modification on the primary microRNA mimics have supported the biogenesis of two individual gene regulating oligonucleotides. Once they were cleaved by the endogenous microprocessor, basal strands and pre-miRNA would be generated inside of cells. Primary microRNA mimics with the basal single strands modified with Antisense oligonucleotides(ASOs) targeting survivor motor nuron2(SMN2-ASO) or miR21(anti-miR21) showed an improved in vitro activity and resulted simultaneous multimodal gene regulation in cells. Combinatorial use of different class of oligonucleotide therapeutics using artificial pri-miRNA may show synergies in therapeutic effect and broad range of applications for gene therapy. |
| 저자 |
김현숙, 이혁진, 장보라
|
| 소속 |
이화여자대 |
| 키워드 |
Nucleic acid nanostructures; RNA motif; Artificial primary miRNA; Multimodal gene regulation |
| E-Mail |
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