| 초록 |
Capillary electrophoresis on microchip-based genetic analysis systems are very useful because of rapid analysis, tiny sample/reagent consumption, and portability. Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is one of promising genotyping method due to its simplicity and versatility. Any DNA sequence variation theoretically cause shift in the mobility and can be detected upon electrophoresis in non-denaturing condition. However, the most critical limitation of CE-SSCP on microchip is low separation resolution resulting from short separation channel length, thus its application had been limited. We investigated the relationship between the resolution of SSCP and the channel width. We translated CE-SSCP system into a microchip format and improved separation resolution via microchannel width control. A high-resolution microchip CE-SSCP system was demonstrated by using an optimum condition (300μm channel width with PEO-PPO-PEO triblock copolymer). |
