| 학회 |
한국공업화학회 |
| 학술대회 |
2014년 가을 (11/12 ~ 11/14, 엑스코(대구)) |
| 권호 |
18권 2호 |
| 발표분야 |
나노-포스터 |
| 제목 |
Signal amplification of a micro-arrayed polydiacetylene(PDA) biosensor using enzyme-catalyzed precipitation |
| 초록 |
Herein, we demonstrate a signal enhancement strategy to improve the sensitivity of an antibody-based biosensor that uses polydiacetylene (PDA) liposomes to detect a target protein (human immunoglobulin E [hIgE]). In order to achieve ultrasensitive detection, multiple siumil applied to PDA biosensor chips offer a signal amplification method that combines the primary immune raction between antigen and antibody with the sandwich method of polyclonal antibody (pAb) –conjugated horseradish peroxidase (HRP). In the second step, fluorescence is enhanced by the mechanical pressure from the precipitate formed by enzyme catalysis. This study explain that increasing the external mechanical force applied to PDA liposomes by enzyme-catalyzed precipitate formation enhanced the sensitivity of the PDA liposome biosensor chip. This strategy can be applied to the detection of other biomolecules in experimental or clinical settings where ultrasensitive and highly specific biosensing is required. |
| 저자 |
이종욱, 심상준
|
| 소속 |
고려대 |
| 키워드 |
Biosensor; Polydiacetylene; hIgE; Signal amplification
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| E-Mail |
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