| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
MALDI-TOF-MS based quantitative analysis technique for N-acyl homoserine lactones as quorum sensing modulators |
| 초록 |
As the AHLs are used for antibacterial drug development and cell-to-cell communication, these are important secondary metabolites. Here, we proposed a highly sensitive and reliable MALDI-MS based 3-oxo AHL quantitation method, which ketone group of AHLs were derivatized with Girard’s reagent T to (GT) to produce a permanent cationic charge state [M]+. By the chemical derivatization of 3-oxo AHLs, its detection sensitivity was improved and the derivatization also allowed accurate quantitative analysis from the PAO1 culture supernatants. This method could be applied for developing high-throughput and sensitive detection methods of quorum sensing signal molecules. |
| 저자 |
이재승1, 김윤곤1, 김성민1, 송원석2, 조성현1, 전효진1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
homoserine lactones; MALDI-TOF-MS; Girard’s reagent T; Quorum sensing
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| E-Mail |
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