| 초록 |
Polymersomes composed of hydrophilic cores and hydrophobic shells have significant storage capacity, providing the synergistic effects between various with different pharmacokinetics and therapeutic mechanisms. To formulate the biodegradable polymersomes, amphiphilic mPEG-b-PLA were first synthesized by ring-opening polymerization with varying the mass fraction of mPEG. In aqueous medium, mPEG-b-PLA self-assembled to form double-layered vesicles at 0.3 of fmPEG. The mPEG-b-PLA-based polymersomes (Psomes) were then prepared using the TFH method. For the controlled releases of gene (pEGFP) and drug (DOX), they were respectively incorporated into the core and the shell of Psomes. pEGFP and DOX-loaded Psomes exhibited distinctly different release profiles, attributed to their loading positions and their distinct encapsulation mechanisms. These results demonstrate that the co-delivery of gene and drug using Psomes could achieve a synergistic effect compared to individual treatments of single gene and/or drug. |
