| 초록 |
The skeletal myocyte can be greatly affected to differentiate by their surrounding topographical structure. To improve myogenic differentiation, we fabricated cell culture platform that sphingosine-1-phosphate (S1P) which regulated myocyte behavior is immobilized on a biomimetic nanopatterned polyurethaneacrylate (PUA) substrate using 3,4-dihydroxyphenylalanine (L-DOPA) for providing topographical and biological cues synergistically. In the present study, we hypothesized that cultured C2C12 cells can be induced to synergistically promote myogenic differentiation on nanopatterned PUA-L-DOPA-S1P. We confirmed that nanopatterned PUA-L-DOPA-S1P showed high hydrophilicity with a suitable range of water contact angle and well-oriented, organized myodubes formation with greater expression of myogenic regulatory factors such as MyoD and MyoG comapred to flat PUA groups. |
