| 초록 |
Hydrated conductive hydrogel that has uniform patterns is a promising approach for tissue regeneration. Herein, we fabricated a hybrid hydrogel by incorporating PEDOT and PSS within the micropatterned PEG hydrogel. This resultant hydrogel provides electrical cues and topological cues simultaneously. It is necessary to immobilize peptides on the hydrogel for the attachment of C2C12 myoblasts. The surface properties such as pattern size, PEDOT particle formation and roughness were carried out through SEM, AFM and conductivity was measured by 4-probe. We also demonstrated that the hydrogel has good biocompatibility through Live/dead assay and MTT Assay. Cellular orientation and cellular differentiation can be identified by staining with FITC-phalloidin and myogenic differentiation markers, MHC and Troponin T respectively. Quantitative analysis of differentiation was conducted by PCR. Overall results showed that this hydrogel has chances for the further studies of tissue engineering. |
