| 초록 |
Most of the bacteria have been evolved to maximize growth rate with fast consumption of carbon sources from surroundings. However, fast growing phenotype featured overproduction of acetate. There exist several glucose uptake pathways in E. coli. As the glucose transporters were mutated, the growth rate and glucose uptake decreased but acetate overflow was relieved and improved biomass was achieved. Transcriptome analysis was implemented to investigate the change between wild type and mutant systematically. Various transcriptional and metabolic transition were identified in mutant including central metabolism, TCA cycle, alternative transporters, quorum sensing, chemotaxis, flagella synthesis and stress induced response. We attempted to construct robust host strain by introducing several value-added compound (EGFP, γ-aminobutyrate, lycopene) pathway to sugar transporter mutants. Eventually, the specific production yields of above compounds were improved in mutants. |
