| 초록 |
13C-MFA(Metabolic Flux Analysis) is a technique for decoding fluxes of central carbon metabolism using C source marked with 13C. Provide the strain with a C source marked 13C and measure the label information of the metabolite. It is then possible to track label information based on models that transform C sources using specific enzymes in strains to decipher flux. Resveratrol is a beneficial compound found in plants. It has effects such as cancer, antioxidant, and life extension. RESV can be made with AcCoA, from the EMP, and Tyr, from the PPP. However, that roads have different properties, making it difficult to make them from one strain. Thus, an attempt was made to produce RESV using co-culture. In this study, two E. coli were designed to supply more precursors of RESV based on previous studies. Then we will produce RESV with co-culture. Finally, using 13C-MFA, the metabolic flux of co-culture will be analyzed, and the method of increasing RESV will be discussed. |
