| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
여성연구자 특별 심포지움 (일반구두발표(Post-Doc., 발표 20분) 및 대학원생 구두발표(발표 15분)만 신청 가능)* |
| 제목 |
Repeated tensile stimulus for mature myotube formation on gelatin nanofiber |
| 초록 |
Volumetric muscle loss (VML) that cannot recovered by native repair system in the body requires surgical intervention such as autologous tissue implantation. In order to develop the scaffold for VML defect site, gelatin nanofiber was developed that co-axially electrospun poly (ε-caprolactone) (PCL) and gelatin nanofiber was treated with 2% (v/v) glutaraldehyde for core gelatin crosslinking, and shell PCL was discarded by solvent etching. Physicochemical property of pure gelatin nanofiber revealed 215±60 nm of average diameter, 6-folds of water swelling ratio, and 19% (w/w) of degradation for 30 days. Mouse skeletal muscle cell line, C2C12s on the nanofiber were differentiated with gradual increasing the nanofiber stretching rate, every day. Myotube related genes were all over-expressed than the cells on non-stretched nanofiber as the result of qPCR and microarray. The results suggest the possibility of gelatin nanofiber with myotube implantation in VML defect site. |
| 저자 |
손영주, 유혁상, Mao Wei, 이솔, 신지운, 이주원
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| 소속 |
강원대 |
| 키워드 |
nanofiber; gelatin; myotube; soft tissue regeneration; electrospinning
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| E-Mail |
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