| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Conjugated Polymer as a Candidate Carrier for Systemic Cancer Treatment using siRNA Therapeutics |
| 초록 |
Small interfering RNA (siRNA) directs the sequence-specific degradation of mRNA through RNA interference (RNAi). siRNA delivery has attracted for cancer treatment. Herein, we used a low molecular weight polyethylenimine (PEI) conjugated with deoxycholic acid (DA). A DA-PEI conjugate was further modified with 4-fluorothiophenol (4-FTP) (4-FTP-DA-PEI) to enhance systemic siRNA delivery. The thiophenol group would mediate a disulfide bonds between the polymer chains and siRNA modified with free thiols (thiol-siRNA) to form and π-π interactions between the aromatic groups and coprostane ring of the bile acid. The stabilized TP-DA-PEI/thiol-siRNA shows improved cellular uptake, serum stability, and transfection efficiency. Furthermore, it shows high accumulation of TP-DA-PEI/thiol-siRNA polyplexes and significant tumor growth inhibition effect in tumor-bearing mice after systemic administration. TP-DA-PEI conjugate could be considered as a promising carrier for systemic cancer therapy. |
| 저자 |
박채은, LYU SIYAN, 임수연, 김다훤, 황위보, 김병덕, 정지훈
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| 소속 |
성균관대 |
| 키워드 |
small interfering RNA; deoxycholic acid; stabilized polyplex; disulfide cross-linking; systemic gene therapy
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| E-Mail |
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