화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2013년 봄 (04/24 ~ 04/26, 광주 김대중컨벤션센터)
권호 19권 1호, p.381
발표분야 생물화공
제목 Identification and quantification of tar compounds in the purification process of paclitaxel from plant cell cultures of Taxus chinensis
초록 In this study, the tar compounds derived from the plant cell cultures of Taxus chinensis were first identified and quantified via GC/MS (gas chromatography/mass spectrometry) and GC (gas chromatography). 2-Picoline, 2,5-Xylenol, Acenaphthene, 1-Methylnaphthalene and o-Xylene were found as major main tar components  in  biomass. These compounds were identified and confirmed by comparing their retention times with those of authentic compounds. Each compound also spiked with pure standard. The contents of 2-Picoline, 2,5-Xylenol, Acenaphthene, 1-Methylnaphthalene, and o-Xylene in biomass were 0.251, 0.159, 0.124, 0.094 wt% and 0.053 wt%, respectively. In liquid-liquid extraction, adsorbent treatment tar was removed 41.62, 89.10%, respectively. After hexane precipitation, all of tars were successfully removed.
저자 김건중, 박규연, 김진현
소속 공주대
키워드 plant cell culture; paclitaxel; tar compounds; identification; quantification
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