| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Application of Modified Target Moiety Chitosan-graft-Branched Poly(Ethylene imine) Copolymer its potential as a gene carrier |
| 초록 |
In this study, we conjugated low molecular water-soluble chitosan(LMWSC) and with high gene expression efficiency Poly(Ethylene imine)(PEI) To enchanced the low tranfection efficiency of chitosan and reduced the high cytotoxicity of PEI and phased in RGD target system. The physicochemical characterisitc of gene carrier was investigated by 1H-NMR. Particle size distribution of the RGDLMPEI/pDNA complexes nanoparticles measured using by the dynamic light scattering(DLS) technique. According the MTT assay of the RGDLMPEI cytoxicity were measured. Gel electrophoresis of the RGDLMPEI/pDNA was confirmed that complex the DNA.We have potential as gene carrier to a variety of experiments were performed for target efficiency, low toxicity and high transfection efficiency. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded the Ministry of Education, Science and Technology (NRF-2011-0012039). |
| 저자 |
김영민, 김남홍, 변지예, 박성철, 박영훈, 나재운, 장미경
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| 소속 |
순천대 |
| 키워드 |
RGD; Target; chitosan; Gene carrier
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| E-Mail |
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