| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
In Vivo Three Dimensional Chitosan Scaffold for Rat Bone Marrow Derived Mesenchymal Stem Cells |
| 초록 |
We herein formulated and characterized an in situ-forming chitosan gel consisting of chitosan and glycerol phosphate disodium salt (GP), and examined its use as an in vivo scaffold for rat bone marrow derived mesenchymal stem cells (rBMSCs). First, the phase transition behaviors of chitosan solutions formulated with and without GP were characterized as a function of temperature. Chitosan solutions containing ＞ 20 wt% GP became a gel at 37 ℃ and maintained this form for 28 days in vitro and in vivo. Next, we examined whether the chitosan gel could act as a suitable biocompatible substrate for the attachment and proliferation of rBMSCs. Immunohistochemistry clearly demonstrated that rBMSCs survived well on the scaffold created by in situ-forming chitosan gel in rats. Our results collectively show for the first time that chitosan gel could serve as an in situ-forming gel scaffold for entrapped rBMSCs in vivo. |
| 저자 |
김경숙1, 안현희1, 이정화1, 이주영1, 김문석1, 이봉2, 강길선3, 이해방1
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| 소속 |
1한국화학(연), 2부경대, 3전북대 |
| 키워드 |
chitosan; rat bone marrow derived mesenchymal stem cells
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| E-Mail |
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