| 초록 |
Recent advances in developmental biology have shown that chemical and physical gradients are common in vivo. Such gradients affect a host of cell behavior such as motility, migration, signaling, and differentiation. To investigate these phenomena in vitro and in vivo, biomedical matrices with physical or biological gradient need to be developed. Until now, several complicated methods have been carried out to produce hydrogels with different stiffness ranges. In this study, we prepared polyvinyl alcohol (PVA)/hyaluronic acid (HA) cylindrical hydrogel with stiffness gradient along the longitudinal direction using a simple LN2-contacted freezing and thawing method. The morphology, stability, mechanical properties, and differentiation behavior of bone marrow stem cells along the longitudinal direction of the prepared PVA/HA cylindrical hydrogel were investigated [This work was supported by the National Research Foundation of Korea (2012-0001052)] . |
