| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
A Study on the Substrate Binding of Poly(hydroxybutyrate) Depolymerase by 2D IR COS |
| 초록 |
Poly(hydroxybutyrate)(PHB) depolymerases have the substrate binding domain (SBD) which binds onto the surface of poly[(R)-3-hydroxybutyrate](P(3HB)) film. In this study, we investigated the binding between SBD of PHB depolymerases and PHB surface using two-dimensional infrared correlation spectroscopy (2D IR COS). Variation in water uptake in SBD on the PHB film was employed as a perturbation to generate a gradient in IR spectra of SBD and PHB. 2D IR COS simplified the overlapped peaks attributed by side chain residues of SBD and enhanced the spectral resolution. The cross peaks in the 2D correlation synchronous spectrum indicated the amino acid residues with hydrophilic side chains, such as asparagines and glutamines, have interactions with carbonyl groups in PHB, and positively charged residues also seem to be involved in the interactions. 2D IR correlation spectra of SBD immobilized on PHB were compared to the spectra of the SBD only solid film. |
| 저자 |
박원민1, 박태정1, 정영미2, 박호석1, 허윤석3, 이상엽1, 홍원희1
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| 소속 |
1한국과학기술원, 2강원대, 3Cornell Univ. |
| 키워드 |
PHB; PHB depolymerase; SBD; 2D IR COS
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| E-Mail |
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