| 학회 |
한국공업화학회 |
| 학술대회 |
2009년 봄 (04/15 ~ 04/17, 호텔인터불고(대구)) |
| 권호 |
13권 1호 |
| 발표분야 |
콜로이드계면화학 |
| 제목 |
Signal Amplification by Forming a Micellar Complex of Cationic Conjugated Polyelectrolyte and Nonionic Surfactants for Optimized FRET to Dye-labeled DNA |
| 초록 |
A polymer-surfactants micellar complex was studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, optical properties of cationic poly(fluorene-co-phenylene) (c-PFP) were increased by nonionic surfactants. TEM study shows the formation of a nanowire micellar complex of c-PFP and surfactants. About 400% enhancement of the FRET signal was measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification was modulated using different nonionic surfactants which perturb the complexation, fine-structure of the complex and the energy transfer. |
| 저자 |
우한영 |
| 소속 |
부산대 |
| 키워드 |
Polyelectrolyte; FRET; DNA
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| E-Mail |
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