| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Controlling adhesion and proliferation of human fibroblasts on ligand-modified alginate hydrogels |
| 초록 | Polymers have attracted much attention to date as a scaffolding material for tissue engineering, as polymer scaffolds can mimic many roles of extracellular matrices in the body.1 While there are many interaction sites available in extracellular matrices that specifically respond to cell receptors, the Arg-Gly-Asp (RGD) sequence has been extensively exploited aimed at improving the understanding of ligand-receptor interactions.2 In this study alginate was chemically modified with a peptide containing the RGD sequence, and mixed with calcium sulfate to form gels.3 Human primary fibroblasts were seeded on the alginate gels, and their adhesion and proliferation was investigated in vitro. Adhesion and spreading of fibroblasts on the RGD-modified alginate gels were significantly enhanced, as compared with those on unmodified alginate gels (Fig. 1). The growth rates of fibroblasts also increased from 3.6 ± 0.7 day-1 (unmodified alginate) to 11.4 ± 0.7 day-1 (RGD-modified alginate). These results clearly indicate that adhesion ligands (i.e., RGD peptide) coupled to synthetic extracellular matrices (i.e., alginate gels) can control adhesion and proliferation of human fibroblasts. (a) (b) Fig. 1. Photomicrographs of human fibroblasts adherent to (a) unmodified and (b)RGD-modified alginate gels. Original pictures were taken after three days of culture. References (1) K. Y. Lee and D. J. Mooney, Chem. Rev., 101, 1869 (2001). (2) C. A. Buck and A. F. Horwitz, Annu. Rev. Cell Biol., 3, 179 (1987). (3) E. Alsberg, K. W. Anderson, A. Albeiruti, R. T. Franceschi, and D. J. Mooney, J. Dental Res., 80, 2025 (2001). |
| 저자 | 배민수1, Mooney2, 이근용1 |
| 소속 | 1한양대, 2Harvard Univ. |
| 키워드 | adhesion ligand; alginate gel; fibroblast; phenotype |
