| 초록 |
Global DNA methylation is in the spotlight as a diagnostic strategy for several severe diseases. However, conventional methods entail laborious work, high costs, and high concentrations at a nanomolar range. We developed a new method based on SERS to detect global DNA methylation. We first used a DNA backbone with well-defined distances between different mCpG sites to study the link between plasmon coupling and a SERS signal. The plasmon shift and SERS enhancement were monitored by plasmon coupling with different mCpG spacing on a genomic DNA backbone. The detection limits for global methylated DNA on the platform of glass and the silver nanowire were 195 fg/ml and 18 fg/ml, respectively. The approach of aligning gold nanoparticles on methylated DNA could be applied for biomolecular templates that can assemble themselves, and a proportional correlation between the concentration and SERS intensity could allow for a quantitative assay of DNA methylation from various samples. |
