| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
PEG-b-Poly(L-lysine-co-L-Phenylalanine)s Conjugated with targeting moieties as a Non-viral Gene Delivery Vector |
| 초록 |
The main obstacle in gene therapy is the development of efficient, non-toxic delivery carriers, which effectively transport DNA inside the nucleus of the target cells. In this study, Lysine based amino acid copolymers containing 30% of phenylalanine residues were prepared with α-methoxy-ω-aminoPEG block at one end. Primary amino groups in the side chain of lysine residues were conjugated with L-arginine derivatives. Obtained copolymers were applied as a gene delivery carrier and the selective targeted delivery to the neuron cells were tested with the introduction of a neuron cell specific peptide by physical mixing the peptides with the copolymers and their transfection efficiency as well as cytotoxicity were tested. PEG-b-Poly(L-lysine-co-L-Phenylalanine)s conjugated with histidine derivatives were also prepared and their transfection efficiency as well as cytotoxicity were tested to investigate the role of the histidine derivatives as endosomal escape moieties. |
| 저자 |
임미정1, 안철희1, 이민형2
|
| 소속 |
1서울대, 2한양대 |
| 키워드 |
gene delivery; PEG-b-Poly(L-lysine-co-L-Phenylalanine)s
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| E-Mail |
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