| 초록 |
Mitochondria are crucial subcellular organelles regulating apoptotic cell death, calcium storage, and ATP synthesis in living cells and have been considered as a subcellular target for gene therapy because they have own DNA and their dysfunctions and malfunction cause lots of human diseases. In this study, triphosphonium-b-poly(ε-caprolactone)-triphosphonium (TPCL1) polymers were designed using for mitochondria-targeting gene delivery. The transfection efficiencies of TPCL1/pDNA complexes were increased with increasing their C/A (cation/anion) ratio and then were reached at a saturated level in HepG2 and MCF7 cells. Also, TPCL1/pDNA complexes exhibited comparable transfection efficiency to bPEI25kDa/pDNA complexes (N/P 5). Especially, TPCL1/pDNA complexes (C/A 2) represented more sustained gene expression and higher mitochondrial targeting than bPEI25kDa/pDNA complexes. In conclusion, TPCL would be a potential gene carrier for mitochondria-targeting sustained gene therapy. |
