| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.466 |
| 발표분야 | 생물화공 |
| 제목 | Data Reconciliation of GC/MS based Flux Distribution in Large-scale Metabolic Flux Analysis |
| 초록 | Various techniques and strategies were developed for the identification of intracellular metabolic conditions, and the gas chromatography/mass spectrometry (GC/MS) based flux analysis has been highlighted in these days. In this study, the combination of isotope based flux data with MFA analysis was examined as a new strategy allowing more accurate quantification of intracellular flux distribution in large metabolic system. To impose GC/MS based data into large metabolic network and obtain optimum flux distribution profile, data reconciliation procedure was applied. As a result, reconciliation of isotopic flux data in large scale MFA is introduced for studying whether experimental isotopic flux data are consistent with optimized flux data based on linear programming. Acknowledgement This work was financially supported by the Advanced Backbone IT Technology Development Project (IMT2000-C3-1) of the Ministry of Information and Communication (MIC). Hardware support by the IBM-SUR program is greatly appreciated. |
| 저자 | 김태용1, 홍순호2, 이상엽1 |
| 소속 | 1한국과학기술원 생명화학공학과, 2대사 및 생물분자공학 연구실 |
| 키워드 | Data reconciliation; metabolic network analysis; metabolic flux analysis; isotopic flux data; Escherichia coli. |
| 원문파일 | 초록 보기 |
