| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 | 9권 2호, p.2439 |
| 발표분야 | 에너지/환경 |
| 제목 | Hydrogen production in the S-deprivation condition using Chlamydomonas reinhardtii : effects of culture parameters |
| 초록 | The objective of this work is to optimize hydrogen production using the green algal Chlamydomonas reinhardtii in the sulfur deprivation condition. To produce hydrogen by the green algae is required hydrogenase activation. But hydrogenase having severe O2 sensitivity are inactivated by photosynthetic O2 evolution. This problem can overcome to separate between the simultaneous photosynthetic O2 and H2 gas evolution(two-stage) by the sulfur deprivation in medium. Two-stage process happen first photosynthetic O2 evolution and carbon accumulation(stage 1), followed H2 evolution by consumption of endogenous substrate when the green algae are incubated under anaerobic condition(stage 2). The experimental method is first to cultivate cells in TAP medium and followed to make the sulfur deprivation condition after washing 5-6 times with TAP-S medium. After 30 h of incubation in the sulfur deprivation, the O2 evolution activity by photosynthesis falls below the O2 uptake activity by respiration. Sulfur deprivation algal cultures evolved hydrogen gas for 3 days under continuous illumination. This work investigated the hydrogen production effect with cell density and residue sulfur concentration in medium. |
| 저자 | 김준표, 심상준 |
| 소속 | 성균관대 |
| 키워드 | hydrogen;hydrogenase;algae;chlamydomonas reinhardtii |
| 원문파일 | 초록 보기 |
