| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 |
22권 2호, p.1801 |
| 발표분야 |
생물화공 |
| 제목 |
Microfluidic single-cell analysis for signaling crosstalk between cell wall integrity and mating pathway in S. cerevisiae |
| 초록 |
Micro-organisms live in continuously changing surroundings and they need to counterbalance with many stresses. In response to the challenges, cells have been developing adaptation mechanisms for maintaining homeostasis. Although the molecular mechanism of cell signaling of single stress becomes revealed, it has not been fully understood how the cells distinguish between multiple stresses. Here, we investigate the signaling crosstalk between cell wall integrity (CWI) and mating pathway of budding yeast via microfluidic device and quantitative single cell analysis. The microfluidic device enables to 1) monitor single yeast cells’ response in media flow by fixing the cells underneath of PDMS micropad, and 2) modulate magnitude of a cell wall stress by dilution of hydrogen. Firstly, we monitor the expression of a mating-specific reporter, based on the Factor Induced Gene 1 promoter driving the expression of Quadruple-Venus fluorescent protein. As a result, we found the activation of CWI signaling inhibits mating signaling, which is shown as decrease of Fig1 expression upon increase of cell wall stress magnitude. |
| 저자 |
이병진1, 정성근1, 진시형1, Ranjan Mishra2, Sung Sik Lee2, Matthias Peter2, 이창수1
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| 소속 |
1충남대, 2ETH Zurich |
| 키워드 |
미생물공학; 바이오칩/나노바이오공학
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
