| 초록 |
Among the many other electrophoretic techniques, capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is one of the promising genotyping method due to its simplicity and versatility. However, the most critical limitation of CE-SSCP on microchip is low separation resolution resulting from short separation channel length, thus its application had been limited. To increase resolution of microchip CE-SSCP analysis system, we tested the relationship between the resolution of SSCP and the channel width. In this study, we translated the CE-SSCP system into a microchip format and improved separation resolution via microchannel width control and found optimum condition (300μm channel width with PEO-PPO-PEO triblock copolymer). This analysis system was successfully applied to the various genetic analyses including food-borne pathogen detection and single nucleotide polymorphism marker detection. |
