| 초록 |
So called organelles are highly optimized to their specific functions of own, and cross-works among them can provide complex cellular metabolism regulation. Whilst there are various kinds of compartments with each of their own specialized function, they all share in common that proteins are used as a major fundamental biological molecule. Information used to synthesize and regulate proteins originates from genes. Thus, the organelles, such as nucleus, mitochondria and chloroplast, which contain genetic materials have a significant role in cellular regulations. The importance of this mechanism, so-called the central dogma, can be applied to metabolism-mimicking synthetic cell designs in the same context. In this study, we created a novel cell-inhalable pseudo-eukaryote-nucleus (PEN) that can produce mRNA transcripts for protein synthesis. PENs consisted of a linearized plasmid, in which genes for proteins are encoded, and a DNA crosslinker. We employed closed envelopes in which a synthetic nucleus was generated simultaneously with the ability to produce mRNA transcripts. |
