| 초록 |
Ethanol production from red macroalgae is a promising alternative liquid biofuel for sustainable energy supply. The budding yeast Saccharomyces cerevisiae is an efficient fermentation microorganism for ethanol production, due to its high ethanol productivity, high tolerance to toxic compounds and robust growth. However, S.cerevisiae has several limitations for utilization of red macroalgae as a feedstock due to 1) low efficient saccharification to convert polymeric-agarose into its monomeric sugars such as D-galactose and 3,6-anhydro-L-galatose(AHG) and 2) incapability to metabolize AHG. These limitations largely decrease the efficiency of utilization on red macroalgae as a feedstock for the production of bioethanol. To access these limitations, we firstly introduced several AHG metabolism pathways as well as agarase in S.cerevisiae. The successful integration of agarase and AHG fermentation pathways in yeast is a critical step towards enabling economic biofuel production. |
