| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Efficient production of 3-hydroxypropionic acid from acetate by engineering metabolic pathway and optimizing carbon flux at malonyl-CoA in Escherichia coli |
| 초록 |
Acetate is an abundant and potential feedstock for microbial processes where diverse value-added chemicals are produced. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate by engineered Escherichia coli. We initially introduced mcr (malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, acetyl-CoA sythetase was overexpressed for improving assimilation of acetate, and iclR (glyoxylate shunt pathway repressor) was deleted for accelerating acetate consumption. Furthermore, we controlled carbon flux at malonyl-CoA node to prohibit malonyl-CoA from leaking to fatty acid synthesis cycle by adding cerulenin. Subsequently, we found that decreased carbon flux to fatty acid synthesis leads dramatic improvement of 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). This result demonstrate that acetate can be utilized as a promising carbon source for microbial process and 3-HP can be produced from acetate with high yield (44.6% of the theoretical maximum yield). |
| 저자 |
이지연, 차상학, 이지훈, 강채원, 이건민, 임현규, 정규열
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| 소속 |
POSTECH |
| 키워드 |
metabolic engineering; 3-hydroxypropionic acid; acetate
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| E-Mail |
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