| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
A Novel Reverse Gene Transfer for Neonatal Cardiomyocytes |
| 초록 |
Cardiomyocytes are difficult to transfer the genes with standard protocols using commercial reagents unless viral vectors are used. In this study, we developed a safe and efficient technique for gene transfer and expression screening in cardiomyocytes with an atelocollagen complexed with nanoparticle and reporter gene. The nanoparticles containing reporter gene, pCMV-DsRed1, were embedded on atelocollagen coated culture plate. Cardiomyocytes were isolated from neonatal rat and seeded on the coated culture plate. The expression of red fluorescent protein (RFP) were confirmed by fluorescent microscopy and quantified by flow cytometry analysis. The DNA complexes immobilized on the collagen surface were efficiently transduced into cardiomyocytes without a significant cytotoxicity. These results suggest that this novel technique using atelocollagen and nanoparticle may be appropriate for general use for gene therapy in cardiomyocytes. |
| 저자 |
차혜련1, 이상준2, 정서연1, Chau Nguyen1, Hieu Quang1, 김용숙3, 권진숙3, 정명호3, 안영근3, 박인규1
|
| 소속 |
1전남의대 의생명과학과 / 전남대병원 심장질환 치료기술 개발 특성화 센터, 2전남의대 의생명과학과 / 전남의대 임상백신연구개발사업단, 3전남대병원 심장질환 치료기술 개발 특성화 센터 |
| 키워드 |
Reverse gene transfer; cardiomyocytes; atelocollagen; nanoparticles
|
| E-Mail |
|
