| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Label-free Detection through Ligand Immobilization on Polydiacetylene-coated and SERS-encoded Beads |
| 초록 |
Multiplex protein detection technologies can obtain a better understanding of protein–protein interactions. However, high-throughput detection is difficult owing to the requirement for detect protein binding event using labeled secondary proteins. We have already determined that polydiacetylene-coated surface-enhanced Raman scattering-encoded beads (PDA–SERS beads) can offer an increased SERS signals and label-free detection through the PDA payer. In here, we successfully introduced a ligand immobilization method on PDA–SERS beads. The color of PDA–SERS beads is changed by upon exposure to external stress and SERS signals could be utilized for identification, indicating their potential for multiplex analyses. By using biotin-conjugated PDA-SERS beads, fluorescence properties were altered by specific binding of streptavidin concentration. In addition, we could detect 2ⅹ10-8 M of streptavidin using the fluorescence intensity except an additional labeling step. |
| 저자 |
김형모, 하유나, 함은일, 강은지, 김태용, 전봉현
|
| 소속 |
건국대 |
| 키워드 |
Silica-coated magnetic nanoparticles; Stöber method; Oleate-MNPs; Gold nanoparticle; Light scattering
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| E-Mail |
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