| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Total N-glycan analysis technique: MALDI-MS-based quantitative targeted glycomics (MALDI-QTaG) |
| 초록 |
In this study, MALDI-MS based quantitative targeted glycomics (MALDI-QTaG) was developed for monitoring the abnormal glycosylation of clinical and biopharmaceutical products. MALDI-QTaG was proposed for sensitive and quantitative analysis of total N-glycans. The derivatization reactions dramatically increased the linearity and sensitivity relative to underivatized glycans. In addition, the analytical strategy was chromatographic purification-free and non-laborious process accessible to the high-throughput analyses. This MALDI-MS based glycomics technology has wide application in many clinical and bioengineering fields requiring sensitive, quantitative and fast N-glycosylation validation. |
| 저자 |
박한규1, 황철환1, 안다희1, 정윤이1, 김성민1, 송원석2, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
N-glycan; MALDI-MS; UPLC-FLR; Chemical derivatization
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| E-Mail |
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