| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 | 13권 2호, p.1681 |
| 발표분야 | 생물화공 |
| 제목 | Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation |
| 초록 | The L-valine producing strain of Escherichia coli was constructed by rational metabolic engineering and stepwise improvement based on transcriptome analysis and in silico gene knock-out simulation. The VAMF strain (Val ΔaceF Δmdh ΔpfkA) harboring pKBRilvBNCED and pTrc184ygaZHlrp was able to produce 7.55 g/liter L-valine from 20 g/liter glucose, resulting in a high yield of 0.378 g L-valine per g glucose. The approaches described here can be a good example of systematically engineering strains for the enhanced production of amino acids. [This work was supported by the Korean Systems Biology Project of the Ministry of Science and Technology (M10309020000-03B5002-00000). Further supports by the LG Chem Chair Professorship and KOSEF through the CUPS are appreciated]. |
| 저자 | 박진환1, 이광호2, 김태용1, 이상엽1 |
| 소속 | 1한국과학기술원, 2한국과학기술원 / CJ 바이오(연) |
| 키워드 | E. coli; L-valine; transcriptome analysis; in silico analysis |
| 원문파일 | 초록 보기 |
