| 초록 |
Cell-penetrating peptides (CPPs) are believed to originate from the protein-transduction domain or membrane trans-locating sequence of viruses, and are well known to deliver biomolecules effectively into a cell. In general, this process requires a specific cellular uptake mechanism so as to not induce damage to the cell membrane. Toward this end, we conjugated CPPs on the surface of polyamidoamine (PAMAM) dendrimers in order to develop a novel nano-carrier with improved cellular uptake and subsequent transfection for gene delivery. PAMAM generation 4 (G4) was conjugated with CPPs consisting of a lysine-phenylalanine-phenylalanine amino acid sequence at altered positions (KFF, FFK). We performed the transfection efficiency and cytotoxicity assay in HeLa and HepG2 cell lines. These results suggest that our nano-carrier will be a promising non-viral vector to delivery therapeutic genes owing to its improved cellular uptake mechanism. |
