| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Multiple approaches based on synthetic biology for engineering Escherichia coli to produce coenzyme B12 |
| 초록 |
Coenzyme B12 is one of the largest and the most structurally complicated chemical in nature. Due to its complex structure, B12 can be obtained only from native producers such as Pseudomonas denitrificans and Klebsiella pneumoniae. However, a lack of genetic engineering tool for these microorganisms limits us to further develop B12 high producer. Rather than engineering native producers, reconstruction of B12 pathway in genetically well-known microorganisms such as an Escherichia coli could be more conceivable to achieve high production. In this study, we applied multiple synthetic biology based approaches in E. coli. Initially, the genes for B12 biosynthesis in P. denitrificans were introduced; the genes were expressed under a strong promoter and optimized 5' untranslated regions (5’-UTR) for maximum expression. Then, an amplification of precursor pool and developing an efficient screening device allowed further strain improvement for increased B12 production. |
| 저자 |
노명현1, 임현규1, 박성훈2, 정규열3
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| 소속 |
1POSTECH 화학공학과, 2부산대, 3POSTECH 화학공학과 / POSTECH 시스템생명공학부 |
| 키워드 |
Coenzyme B12; Synthetic biology; Metabolic engineering
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| E-Mail |
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