| 초록 |
1,3-propanediol (1,3-PDO) is an industrially important chemical with increasing demand as an intermediate to fibers and resins. No natural microorganism can produce 1,3-PDO directly from glucose and only few scientific details on glucose-based 1,3-PDO production were disclosed. This study aims to provide information on glucose-based 1,3-PDO production using Klebsiella pneumoniae J2B as a microbial cell factory which can convert glycerol to 1,3-PDO and synthesize an essential coenzyme B12. K. pneumoniae J2B was engineered to have synthetic pathway which connect glycolytic pathway with that of 1,3-PDO synthesis from glycerol, thus to directly produce the diol from glucose. In addition, glycerol dissimilation pathways were disrupted. Furthermore, the metabolic flux toward 1,3-PDO was improved by modulation of byproduct formation pathway and fulfilling the demand of NADH. The engineered strains successfully produce 1,3-PDO from glucose. |
