| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Novel Hybrid Input Part Using Coenzyme B12 Riboswitch and Transcriptional Repressor for Signal Inverting Amplifier |
| 초록 |
Construction of complex genetic circuits for practical applications requires numerous tunable genetic parts. However, the limited diversity and complicated tuning methods used for the input parts hinders the scalability of genetic circuits. Therefore, a new type of input part is required that responds to diverse signals and enables easy tuning. Here, we developed RNA–protein hybrid input parts that combine a riboswitch and orthogonal transcriptional repressors. The hybrid inputs successfully regulated the transcription of an output in response to the input signal detected by the riboswitch and resulted in signal inversion because of the expression of transcriptional repressors. Fold-change and half-maximal effective concentration were modulated and amplified simply by changing the promoter strength. Furthermore, the hybrid input detected both exogenous and endogenous signals, indicating potential applications in metabolite sensing. |
| 저자 |
황재성, 장성연, 장성호, 노명현, 임현규, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
biosensor; coenzyme B12; riboswitch; input part
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| E-Mail |
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