| 학회 |
한국공업화학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
8권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Expression of DOI synthase (kanA) in Streptomyces lividans TK24 and Comparison with E. coli Counterpart |
| 초록 |
A cluster for the kanamycin (Km) biosynthesis has been isolated from the genomic library of S. kanamyceticus ATCC 12853. Sequencing of 47 kb region or chromosomal DNA revealed 40 open reading frames (ORFs) including Km biosynthetic, regulatory and resistance genes [2]. For expressing actinomycetes genes in Streptomyces spp, an expression vector (pIBR25) was constructed and used to express a gene encoding 2-deoxy-scyllo-inosose synthase (kanA) in S. lividans TK24 from the Km biosynthetic gene cluster. The activity of kanA was dependent on the culture period of the transformants reaching maximum at 72 h. The application of sorbitol and betaine in the expression medium improved the formation of soluble KanA as well as its activity when it was expressed in Escherichia coli. The results provide a valuable tool for expressing aminoglycoside-aminocyclitols (AmAcs) biosynthetic genes in Streptomyces spp. |
| 저자 |
Madan Kumar Kharel, Rajan Lamichhane, 이희찬, 류광경, 송재경, Mai Le Thuy
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| 소속 |
선문대 |
| 키워드 |
DOI synthase; Streptomyces; kanamycin; biosynthesis; expression
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| E-Mail |
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