| 초록 |
Previous studies have shown that the DR1558 gene from Deinococcus radioduransin, it can regulate multistress if introduced into a strain. In this study, the DR1558 was introduced into the engineered strain to confirm the improved production of 1,5-diaminopentane. To express DR1558 in an engineered Corynebacterium glutamicum KCTC 1857 ldcC strain, DR1558 was integrated in to the strain by gene cloning and controlling its expression with a strong synthetic H30 promoter. A control strain was used in which strain DR1558 was not introduced. Recombinant and wild-type strains were compared by qRT-pcr for expression levels of genes in the central pathway.The strain inserted with the DR1558 gene showed more 1,5-diaminopentane production and stable cell growth,and After 48 hours of incubation, the residual lysine was found to be smaller than wild type strain. As a result, we have shown that DR1558 gene can be expressed as a stress-regulated gene in the engineered-strain to produce more targeted products. |
