| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
바이오센서 |
| 제목 |
Biosensor Based on the Selective Aggregation of Gold Nanoparticles |
| 초록 |
We report the detection of specific DNA sequences using selective aggregation of unmodified gold nanoparticles by the hybridization of oligonucleotide. Oligonucleotide sequences were designed to detect the signal-induced proliferation-associated gene 1 (Sipa1) since Sipa1 polymorphism is known to be associated with metastatic process. To monitor selective aggregation of gold nanoparticles, we have used the UV-vis absorption spectroscopy, quasi-elastic light scattering (QELS), and zeta potential measurement. Because ssDNA and dsDNA have different electrostatic properties we were able to selectively aggregate gold nanoparticles for the perfectly matched DNA under optimal salt concentration. Our results indicate that a change in the electrostatic interaction is responsible for the selective aggregation of gold nanoparticles upon the addition of DNA. |
| 저자 |
이강택1, 이윤희1, 김태훈1, 이소영2, 주상우3
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| 소속 |
1연세대, 2서울대, 3숭실대 |
| 키워드 |
DNA hybridization; biosensor; gold nanoparticles; aggregation
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| E-Mail |
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